Western blot. All lanes: TBC1D22A antibody at 18µg/ml. Lane 1: Colo320 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Rat skeletal muscle tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60, 51, 37, 54 kDa. Observed band size: 60 kDa