Western blot. All lanes: OTUD6B antibody at 4µg/ml. Lane 1: PC-3 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: MDA-MB-231 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 34, 22 kDa. Observed band size: 34 kDa