Western blot. All lanes: NELL1 antibody at 3.47 ug/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse kidney tissue. Lane 3: Mouse spleen tissue. Lane 4: Human placenta tissue. Lane 5: Hela whole cell lysate. Lane 6: Jurkat whole cell lysate. Lane 7: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 90, 85 kDa. Observed band size: 90, 65, 58, 35 kDa.