Western blot. All lanes: NUP43 antibody at 4µg/ml. Lane 1: A431 whole cell lysate. Lane 2: HL60 whole cell lysate. Lane 3: THP-1 whole cell lysate. Lane 4: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43, 32 kDa. Observed band size: 43 kDa