Western blot. All lanes: UFD1 antibody at 3.22µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 35, 39, 30 kDa. Observed band size: 35 kDa