Western blot. All lanes: LFNGantibody at 4.43ug/ml. Lane 1: Mouse spleen tissue. Lane 2: K562 whole cell lysate. Lane 3: HL60 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 42, 29, 40, 36 kDa. Observed band size: 42 kDa.