Western blot. All lanes: ASAP3 antibody at 1µg/ml. Lane 1: A549 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 100, 44, 98 kDa. Observed band size: 100 kDa