Western blot. All lanes: MTERF3 antibody at 3.94µg/ml. Lane 1: U251 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 48, 38, 35 kDa. Observed band size: 48 kDa