Western blot. All lanes: FOXRED1 antibody at 5.8µg/ml. Lane 1: A549 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 54, 31, 53 kDa. Observed band size: 54 kDa