Western blot. All lanes: RASSF5antibody at 5.46ug/ml. Lane 1: Thp-1 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 48, 31, 38 kDa. Observed band size: 48 kDa.