Western blot. All lanes: CEP57L1 antibody at 6.26µg/ml. Lane 1: CEM whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 54, 46 kDa. Observed band size: 54 kDa