Western blot. All lanes: ANKS1B antibody at 5.28µg/ml. Lane 1: 293T whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 139, 49, 58, 53, 52, 86, 48, 41, 29, 45 kDa. Observed band size: 139 kDa