Western blot. All lanes: CD276 antibody at 0.48µg/ml. Lane 1: U251 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 34, 53 kDa. Observed band size: 53 kDa