Western blot. All lanes: SFXN4 antibody at 0.3µg/ml. Lane 1: K562 whole cell lysate. Lane 2: U251 whole cell lysate. Lane 3: U87 whole cell lysate. Lane 4: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 38, 35, 25 kDa. Observed band size: 38 kDa