Western blot. All lanes: KCTD9 antibody at 8µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: HT29 whole cell lysate. Lane 4: U251 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43 kDa. Observed band size: 43 kDa