Western blot. All lanes: DDI2 antibody at 4µg/ml. Lane 1: A431 whole cell lysate. Lane 2: HL60 whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Caco-2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45, 24, 47 kDa. Observed band size: 47 kDa