Western blot. All lanes: TBCEL antibody at 12µg/ml. Lane 1: Rat gonadal tissue. Lane 2: PC-3 whole cell lysate. Lane 3: Rat heart tissue. Lane 4: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 49 kDa. Observed band size: 49 kDa