Western blot. All lanes: Acyl-coenzyme A synthetase ACSM3, mitochondrial antibody at 10ug/ml. Lane 1: 293T whole cell lysate. Lane 2: Mouse kidney tissue. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 67, 50 kDa. Observed band size: 67 kDa.