Western blot. All lanes: FBXO7 antibody at 3.97µg/ml. Lane 1: Mouse gonadal tissue. Lane 2: Jurkat whole cell lysate. Lane 3: 293T whole cell lysate. Lane 4: PC-3 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 59, 50, 46 kDa. Observed band size: 59 kDa