Western blot. All lanes: COTL1 antibody at 8µg/ml. Lane 1: Mouse brain tissue. Lane 2: Hela whole cell lysate. Lane 3: Mouse kidney tissue. Lane 4: HepG2 whole cell lysate. Lane 5: Mouse thymus tissue. Lane 6: U87 whole cell lysate. Lane 7: HL60 whole cell lysate. Lane 8: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 16 kDa. Observed band size: 16 kDa