Western blot. All lanes: PDCD1 antibody at 6µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: K562 whole cell lysate. Lane 4: Mouse stomach tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa