Western blot. All lanes: Flotillin-2 antibody at 4ug/ml. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Rat brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 47 kDa. Observed band size: 47 kDa.