Western blot. All lanes: ELF2 antibody at 3.35ug/ml. Lane 1: HL60 whole cell lysate. Lane 2: A549 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: Hela whole cell lysate. Lane 6: Mouse lung tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 64, 63, 57, 58, 55 kDa. Observed band size: 64, 50 kDa.