Western blot. All lanes: HADH antibody at 2.38µg/ml. Lane 1: Mouse heart tissue. Lane 2: Mouse liver tissue. Lane 3: Mouse kidney tissue. Lane 4: Mouse skeletal muscle tissue. Lane 5: NIH/3T3 whole cell lysate. Lane 6: Hela whole cell lysate. Lane 7: 293T whole cell lysate. Lane 8: A431 whole cell lysate. Lane 9: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 35, 43, 37 kDa. Observed band size: 35 kDa