Western blot. All lanes: Cullin-1 antibody at 6ug/ml. Lane 1: PC-3 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 90 kDa. Observed band size: 90 kDa.