Western blot. All lanes: PDK3 antibody at 1.6µg/ml. Lane 1: Mouse kidney tissue. Lane 2: RAW264.7 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 47, 49 kDa. Observed band size: 47 kDa