Western blot. All lanes: ITPK1 antibody at 3.58µg/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 46, 36 kDa. Observed band size: 46 kDa