Western blot. All lanes: Carbonic anhydrase 1 antibody at 2ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 53, 14 kDa. Observed band size: 53 kDa.