Western blot. All lanes: ERVFRD-1 antibody at 3.93ug/ml. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.