Western blot. All lanes: PCDH1 antibody at 2.56ug/ml. Lane 1: A431 whole cell lysate. Lane 2: Human breast cancer cell. Lane 3: Jurkat whole cell lysate. Lane 4: Hela whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 115, 134 kDa. Observed band size: 115 kDa.