Western blot. All lanes: Antigen 85-A antibody at 2ug/ml. Lane 1: Recombinant Mycobacterium tuberculosis Antigen 85-A protein 1ug. Lane 2: Recombinant Mycobacterium tuberculosis Antigen 85-A protein 10ug. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa.