Western blot. All lanes: LCN2 antibody at 2µg/ml. Lane 1: Human positive serum at 1: 10. Lane 2: Human positive serum at 1: 100. Lane 3: Human positive serum at 1: 500. Secondary. Goat polyclonal to rabbit IgG at 1/15000 dilution. Predicted band size: 23 kDa. Observed band size: 23 kDa