Western blot. All lanes: ZNF398 antibody at 3µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: Jurkat whole cell lysate. Lane 5: Caco-2 whole cell lysate. Lane 6: A549 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 72, 53 kDa. Observed band size: 53 kDa