Western blot. All lanes: UCP3 antibody at 0.9µg/ml. Lane 1: Jurkat whole cell lysate. Lane 2: SH-SY5Y whole cell lysate. Lane 3: U251 whole cell lysate. Lane 4: A549 whole cell lysate. Lane 5: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 35, 30, 23 kDa. Observed band size: 35 kDa