Western blot. All lanes: HNRNPD antibody at 2µg/ml. Lane 1: 293T whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 39, 37, 33, 31 kDa. Observed band size: 39 kDa