Western blot. All lanes: UBE2D1 antibody at 2µg/ml. Lane 1: Mouse thymus tissue. Lane 2: NIH/3T3 whole cell lysate. Lane 3: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 17 kDa. Observed band size: 17 kDa