Western blot. All lanes: TMEM68 antibody at 4µg/ml. Lane 1: Mouse thymus tissue. Lane 2: K562 whole cell lysate. Lane 3: U251 whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: MCF-7 whole cell lysate. Lane 6: Jurkat whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 38, 30, 16 kDa. Observed band size: 38 kDa