Western blot. All lanes: TMCC1 antibody at 1µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: Mouse liver tissue. Lane 3: Mouse kidney tissue. Lane 4: HL60 whole cell lysate. Lane 5: K562 whole cell lysate. Lane 6: U937 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 73, 54 kDa. Observed band size: 73 kDa