Western blot. All lanes: TIMM17B antibody at 1.75 ug/ml. Lane 1: U251 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HL60 whole cell lysate. Lane 5: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 19, 24 kDa. Observed band size: 19 kDa.