Western blot. All lanes: TCTA antibody at 1.6µg/ml. Lane 1: Mouse liver tissue. Lane 2: Mouse gonadal tissue. Lane 3: Mouse muscle tissue. Lane 4: HL60 whole cell lysate. Lane 5: U251 whole cell lysate. Lane 6: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 12 kDa. Observed band size: 12 kDa