Western blot. All lanes: STK16 antibody at 12ug/ml. Lane 1: A549 whole cell lysate. Lane 2: Mouse brain tissue. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 35 kDa. Observed band size: 35 kDa.