Western blot. All lanes: SRM antibody at 8µg/ml. Lane 1: PC-3 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 34 kDa. Observed band size: 34 kDa