Western blot. All lanes: SQRDL antibody at 4.67ug/ml. Lane 1: A549 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.