Western blot. All lanes: Socs3 antibody at 12µg/ml. Lane 1: K562 whole cell lysate. Lane 2: A375 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 25 kDa. Observed band size: 25 kDa