Western blot. All lanes: SNCG antibody at 16µg/ml. Lane 1: HT29 whole cell lysate. Lane 2: Mouse brain tissue. Lane 3: 293T whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa