Immunoprecipitating CTSD in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of CSB-PA02129A0Rb in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: CSB-PA02129A0Rb (8µg) + HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (10µg)