Western blot. All lanes: Serpin B5 antibody at 10ug/ml. Lane 1: HepG2 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43, 26 kDa. Observed band size: 43 kDa.