Western blot. All lanes: SEPHS1 antibody at 3.05ug/ml. Lane 1: Mouse liver tissue. Lane 2: HepG2 whole cell lysate. Lane 3: Mouse kidney tissue. Lane 4: U251 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 43, 36 kDa. Observed band size: 43 kDa.