Western blot. All lanes: SCP2 antibody at 2µg/ml. Lane 1: A549 whole cell lysate. Lane 2: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 59, 16, 35, 51, 7, 55, 57 kDa. Observed band size: 59 kDa