Western blot. All lanes: RTKN antibody at 4.66ug/ml. Lane 1: A431 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: MCF-7 whole cell lysate. Lane 4: Mouse brain tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 63, 62, 57 kDa. Observed band size: 63 kDa.